Paul Roberts' Application Blog

Paul Roberts is an HPLC Applications Chemist at Anatune and has been working with the company since 2008. He is responsible for the development of new HPLC and LC/MS solutions and supporting instrument methods that have been developed in the Anatune laboratory. He has experience working in laboratories, both in the UK and Europe, being primarily involved in the determination of emerging contaminants in the environment.
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25th Jun 2010
PAHs from Soil

I've been working on the extraction of PAHs from soils again this week. We have a fairly crude method whereby we extract 2 grams of soil in conjunction with 1g of copper. To the soil sample the Gerstel Multi Purpose Sampler (MPS) adds 2ml of acetone and the soil/acetone slurry is mixed for 15 minutes in an agitator. Then 4ml of hexane is added and the mixture shaken again for 15 minutes.... read more

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18th Jun 2010
Ongoing activities

So I've been working on the Vitamin D methodology this week, we are almost ready to start demoing the system to customers....at the heart of the methodology is a solvent facilitated protein crash, which is working really well. Following the centrifugation step to pelletise the proteins, an aliquot of the supernatant is put through a C8 solid phase extraction cartridge before injection onto LC-... read more

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10th Jun 2010
New hardware.......

.....So Agilent were here at the end of last week and the beginning of this one, fitting the turbo pump upgrade and multimode ionisation source to allow me to get cracking with the Vitamin D work, we are looking to automate the protein precipitation of serum samples and then automate the SPE coupled to LC-QQQ....

......Vitamin D regulates the calcium and phosphorus levels in the blood... read more

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27th May 2010
My Applog Initiation

So this is my first Applog entry and actually my first ever blog entry too, nothing like being thrown in at the deep end....so here goes.....

I've been working on a few things this week;

Firstly I have been working on a method to extract PAH's from soil. It's a fairly crude method using a solvent shake, with some copper and sodium sulphate, and then a... read more

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