GlaxoSmithKline were interested in measuring volatile components from cell lines. This application note details how volatiles can be analysed from a non-volatile matrix using Automatic Tube Exchange (ATEX). ATEX is an automated thermal desorption technique where the sample is inserted into a microvial which in turn is inserted into a Thermal Desorption Tube. Volatiles from the sample are then desorbed onto the GC/MS leaving the involatile material left behind in the tube which keeps the GC inlet clean. Differences in cell lines (two groups) could then be seen by performing Principle Component Analysis (PCA) and key analytes indicative of a particular group were determined.
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Analysis of Human Cell Lines Using Automatic Tube Exchange (ATEX) and GC/MS