The measurement of urinary free cortisol (UFC) is used in the investigation of Cushing’s syndrome, a hormonal disorder caused by prolonged exposure to high levels of this hormone. Only a small percentage of circulating cortisol is biologically active (free), with the majority of cortisol inactive (protein bound). UFC represents excretion of the circulating free cortisol. This application note presents a fully automated method for UFC featuring automated internal standard addition, SPE clean-up and extraction. Sample preparation is coupled directly to LC-MS/MS. The system is capable of running 98 samples in 12 hours and 21 minutes. Sample preparation time is under 5 minutes fitting within the LC runtime ensuring highest possible throughput.
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Automating the Extraction and Analysis of Urinary Free Cortisol Using ITSP, GERSTEL MPS and Agilent LC-MS/MS