Three derivatisation workflows for metabolomics applications were fully automated: MOX-TMS using MSTFA, MOX-TMS using TMSCN and tBDMS.
All three derivatisation methods were successfully and reproducibly performed as shown by the internal standards Areas and RSDs%.
Several components hits were identified by the Agilent Unknown Analysis Software as target TMS and tBDMS derivatives with match factor above 70.
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Automation of Derivatisation Workflows for GC-MS Metabolomics Applications