Metabolomics focuses on the characterisation of small-molecule metabolite profiles (Molecular weight < 2000 Da) specific to investigated pathways. Metabolomics studies usually require the preparation of extensive sample sets to allow significant differentiation between sample types in biological matrices. Hence, analytical data quality (i.e. low analytical variability) is essential to highlight true biological variability.
Fatty acids are small molecules whose presence and abundance are key to many pathways in metabolic regulation. In this study, lipids were extracted from salmon tissue by an automated Folch extraction and the extracts were subjected to direct transmethylation before injection on the GC-MS system.
Very good agreement was achieved between the three replicates (RSD <10 %) and when sampler were extracted using the standards approach and using bead beating.
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Fully automated on-line Folch Extraction and trans-methylation of fatty acids in salmon tissue