Precise extraction and measurement is essential for any method used in metabolomics as significant metabolic effects can be observed as small chemical changes. The measurement of fatty acids is a well-established metabolomic profiling technique. The simplest method for looking at these profiles is to derivatise the sample of interest, trans-esterifying lipids to form the methyl esters of the fatty acids, with a subsequent liquid-liquid extraction (LLE) clean-up step.
Integration of the base ion for each of the methyl esters showed extractions of a sample of oregano gave a mean %cv was 8.2% for the fatty acids present at 1 % or more of the most abundant FAME, methyl hexadecanoate.
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Automated derivatisation and extraction of fatty acids using the MPS and GC/Q-TOF.